Demonstration of cobamide coenzyme in Ascaris suum.
نویسندگان
چکیده
I. Cobamide coenzyme was extracted from the body wall of adult female Ascaris suum maintained in Ellison's medium containing vitamin B11• 2. The identification of the coenzyme in purified fractions was based on (a) specific spectral changes following treatment of the preparation with light and cyanide, (b) coenzyme activity in the glutamate mutase reaction and (c) inactivation of the coenzyme activity by light. 3. A fivefold increase in coenzyme occurre d in worms maintained in Ellison's medium plus vitamin B11, compared to the endogenous value. The data indicate that vitamin B11 is converted by A. suum into the metabolically active coenzyme.
منابع مشابه
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ورودعنوان ژورنال:
- Comparative biochemistry and physiology. B, Comparative biochemistry
دوره 50 3 شماره
صفحات -
تاریخ انتشار 1975